ピックアップ論文

Keyとなる技術に関する成果

Key1：独自の理論計算法による耐熱化変異体予測技術

1. Satoshi Yasuda, Yuta Kajiwara, Yuuki Takamuku, Nanao Suzuki, Takeshi Murata, Masahiro Kinoshita
   "Identification of Thermostabilizing Mutations for Membrane Proteins: Rapid Method Based on Statistical Thermodynamics"
   J. Phys. Chem. B, 120, 3833-3843 (2016). doi:10.1021/acs.jpcb.6b01405
2. Yuta Kajiwara, Takahiro Ogino, Satoshi Yasuda, Yuuku Takamuku, Takeshi Murata, Masahiro Kinoshita
   "Physical origins of remarkable thermostabilization by an octuple mutation for the adenosine A2a receptor"
   Chem. Phys. Lett., 657, 119 (2016).
3. Yuta Kajiwara, Satoshi Yasuda, Yuuki Takamuku, Takeshi Murata, Masahiro Kinoshita
   "Identification of thermostabilizing mutations for a membrane protein whose three-dimensional structure is unknown"
   J. Comput. Chem., 38(4), 211-223 (2017). doi:10.1002/jcc.24673
4. Satoshi Yasuda, Yuta Kajiwara, Yosuke Toyoda, Kazushi Morimoto, Ryoji Suno, So Iwata, Takuya Kobayashi, Takeshi Murata, Masahiro Kinoshita
   "Hot-Spot Residues to be Mutated Common in G Protein-Coupled Receptors of Class A: Identification of Thermostabilizing Mutations Followed by Determination of Three-Dimensional Structures for Two Example Receptors"
   J. Phys. Chem. B, 121, 6341-6350 (2017). doi:10.1021/acs.jpcb.7b02997
5. Yuta Kajiwara, Satoshi Yasuda, Simon Hikiri, Tomohiko Hayashi, Mitsunori Ikeguchi, Takeshi Murata, Masahiro Kinoshita
   "Physical Origin of Thermostabilization by a Quadruple Mutation for the Adenosine A2a Receptor in the Active State"
   J. Phys. Chem. B, 122, 4418-4427 (2018). doi:10.1021/acs.jpcb.8b00443

Patents

1. 特許第6359656
   PCT/JP2015/068277

Key3：結合化合物スクリーニング技術

1. Nanao Suzuki, Yuuki Takamuku, Tomohiro Asakawa, Makoto Inai, Tomoya Hino, So Iwata, Toshiyuki Kan, Takeshi Murata
   "An efficient screening method for purifying and crystallizing membrane proteins using modified clear-native PAGE"
   Anal. Biochem., 548, 7-14 (2018). doi:10.1016/j.ab.2018.02.007
2. Kano Suzuki, Kenji Mizutani, Shintaro Maruyama, Kazumi Shimono, Fabiana L. Imai, Eiro Muneyuki, Yoshimi Kakinuma, Yoshiko Ishizuka-Katsura, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Takeshi Murata
   "Crystal structures of the ATP-binding and ADP-release dwells of the V₁ rotary motor"
   Nat. Commun., 7, 13235 (2016). doi:10.1038/ncomms13235

Patents

1. 特願2019-006819号

Key4：機能性抗体の作製技術

1. Tomoya Hino, Takatoshi Arakawa, Hiroko Iwanari, Takami Yurugi-Kobayashi, Chiyo Ikeda-Suno, Yoshiko Nakada-Nakura, Osamu Kusano-Arai, Simone Weyand, Tatsuro Shimamura, Norimichi Nomura, Alexander D. Cameron, Takuya Kobayashi, Takao Hamakubo, So Iwata, Takeshi Murata
   "G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody"
   Nature, 482, 237-240 (2012). doi:10.1038/nature10750
2. Tomoya Hino, So Iwata, Takeshi Murata
   "Generation of functional antibodies for mammalian membrane protein crystallography"
   Curr. Opin. Struct. Biol., 23, 563-568 (2013). doi:10.1016/j.sbi.2013.04.007

Patents

1. 特許第5526448号

Topick3：耐熱化ロドプシンを利用した新規光電池・バイオセンサーの開発

1. Satoshi Yasuda, Tomohiko Hayashi, Yuta Kajiwara, Takeshi Murata, Masahiro Kinoshita
   "Analyses based on statistical thermodynamics for large difference between thermophilic rhodopsin and xanthorhodopsin in terms of thermostability"
   J. Chem. Phys., 150, 055101 (2019). doi:10.1063/1.5082217
2. Satoshi Yasuda, Tomoki Akiyama, Sayaka Nemoto, Tomohiko Hayashi, Tetsuya Ueta, Keiichi Kojima, Takashi Tsukamoto, Satoru Nagatoishi, Kouhei Tsumoto, Yuki Sudo, Masahiro Kinoshita, Takeshi Murata
   "Methodology for Further Thermostabilization of an Intrinsically Thermostable Membrane Protein Using Amino Acid Mutations with Its Original Function Being Retained"
   J. Chem. Inf. Model., 60(3), 1709-1716 (2020). doi:10.1021/acs.jcim.0c00063

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